Data / GraphCompleted2 min read

Plant Biology Statistical Analytics Pipeline

Statistical Data Science

Plant Biology Statistical Analytics Pipeline

Overview

Conducted a full-cycle statistical analysis pipeline for a PhD researcher in plant biology evaluating plant responses to environmental stressors. The pipeline ingests multi-factorial experimental agronomic data, runs data cleaning and normality testing, and conducts Two-Way ANOVA with Tukey HSD post-hoc tests to identify statistically significant genotype-by-environment interactions. Dimensionality reduction via Principal Component Analysis (PCA) and hierarchical clustering revealed underlying phenotypic groupings and isolated key physiological markers for drought tolerance rankings, delivering publication-ready visualizations and statistical tables.

Problem & Solution

The Problem

Biological research experiments feature complex multivariate interactions across treatments and genotypes, requiring rigorous, reproducible statistical modeling to extract publication-grade insights.

The Solution

Automated statistical pipeline executing Two-Way ANOVA, Principal Component Analysis (PCA), hierarchical clustering, correlation matrices, and drought tolerance ranking indices.

Architecture & Dataflow

Pipeline Architecture
Raw Agronomic Data → Preprocessing & Normalization → Two-Way ANOVA (Statsmodels) → PCA & Clustering (Scikit-learn) → Correlation Matrices → Drought Ranking Index → Publication Figures (Seaborn/Matplotlib)

Technical Stack & Implementation

AI / ML & Models

  • PCA (Principal Component Analysis)
  • Hierarchical Clustering
  • Scikit-learn

Backend & APIs

  • Python

Frontend & UI

  • Seaborn
  • Matplotlib
  • Publication Figures

Data & Storage

  • Two-Way ANOVA
  • Tukey HSD Test
  • Statsmodels
  • SciPy
  • Pandas
  • NumPy

Technical Challenges & Solutions

01

Handling non-normal residual distributions and missing experimental values

02

Disentangling multi-factorial treatment effects across multiple plant cultivars

03

Generating high-resolution publication-quality plots conforming to journal submission standards

Key Highlights & Results

Identified top drought-resistant genotypes with statistically significant markers (p < 0.01)

Reduced 15+ physiological traits into 2 principal components explaining >80% total variance

Outputs directly integrated into doctoral research manuscript and presentations

Two-Way ANOVA with Tukey HSD post-hoc analysis
Principal Component Analysis (PCA) for variance decomposition
Hierarchical cluster analysis of genotype drought tolerance
Multi-trait correlation matrices and interaction heatmaps
Publication-ready statistical figures and summary tables
Reproducible, modular Python data pipeline